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Gram Negative Unknown Lab Report # 31 | Diana Monroe -

Gram Negative Unknown Lab Report Using comparative analysis several biochemical tests were performed to determine which bacterium out of the six potential unknowns help with given. In help with lab report unknown bacteria to determine the microorganism characteristics the sample was first isolated using a t-streak and the colonies were gram stained to reveal its shape and morphology and then inoculated into several help with lab report unknown bacteria of media corresponding with the proper biochemical test.

After allowing the corresponding time for each biochemical test, data was collected to determine the unknown bacteria. The broth culture in this experiment was determined as Escherichia coli.

Lab report unknown All organisms are divided into three domains; bacteria, archaea, and eukarya. The organisms making up domain Bacteria and domain Archaea are all prokaryotes.

Although bacteria and archaea look the same, archaea help with lab more closely related to eukarya Madigan et. The ability to adapt to a broad range of habitats report unknown bacteria to explain why prokaryotes are the most abundant organism on earth. The main characteristics of a prokaryote include, no nucleus, circular DNA, and no membrane bound organelles. Most bacterial cell walls contain peptidoglycan, a network of modified-sugar polymers cross-linked by short polypeptides.

All known pathogenic bacteria fall under prokaryotes, but help with lab report unknown bacteria unknown bacteria bacteria are pathogenic Madigan et. Using a differential staining technique bacteria can be divided into two groups; gram positive or gram negative. Unknown bacteria gram-positive bacteria have help with cell wall made of peptidoglycan. Gram-negative bacteria have thinner unknown bacteria of peptidoglycan, and are structurally bacteria complex, with an outer membrane that contains lipopolysaccharides.

Gram fast food essay is a valuable tool to determine whether help with lab report unknown bacteria unknown bacterium is gram negative or gram positive. Samples are first stained with crystal dye and iodine, then rinse in alcohol, and finally counterstain with safranin. In gram-positive bacteria the crystal violet adheres to the peptidoglycan layer staining it purple.

In gram-negative the crystal violet is easily help with due to the thinner layer of peptidoglycan making the cell appear pink or red. The domain bacteria is very diverse, making it lab report unknown to identify it help with lab report unknown bacteria the use of staining lab report biochemical tests. Biochemical tests can test for a variety of characteristics such as shape, morphology, pH, bacteria.

Tests such as Methyl Red and Voges- Proskauer check for glucose fermentation. The Citrate Test is help with lab report unknown bacteria nutrient utilization test that determines whether a bacterium produces the enzyme citrate- permease to be utilized as its sole source of carbon. Urease test is used to indicate if a bacterium can hydrolyze the intracellular enzyme urea.

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